Synthesis of Nickel Oxide Nanoparticles from Syzygium cumini Plant Fruit Pulp Extract: Study of their Antibacterial, Antifungal and Cytotoxic Activities on CHO Cells

Lalitha Ammadu Kolahalam1,, K.R.S. Prasad1,*,, P. Murali Krishna2,*, and N. Supraja3,

1Department of Chemistry, Koneru Lakshmaiah Education Foundation, Green Fields, Vaddeswaram, Guntur 522502, India

2Department of Chemistry, Ramaiah Institute of Technology (Autonomous Institute, Affiliated to VTU), Bangalore-560054, India

3Nanotechnology Laboratory, Acharya N.G. Ranga Agricultural University, Tirupati-517502, India

*Corresponding authors: E-mail:;


The nickel oxide nanoparticles were synthesized using Syzygium cumini plant fruit pulp in a simple, ecofriendly and cost-effective procedure using the green chemistry route. The prepared nickel oxide nanoparticles were characterized by UV-visible (UV-Vis), Fourier transform infrared (FT-IR) spectroscopy, powder X-ray diffraction spectrometry (XRD), zeta potential (ZE), particle size (DLS), field emission scanning electron microscope (FE-SEM) analysis. The synthesized nanoparticles showed on an average of 16 to 31 nm in size. The synthesized nanoparticles were tested on several Gram-positive and Gram-negative bacteria and also on fungi, which showed less toxicity to them. Finally, the synthesized nanoparticles cytotoxic activity was tested on Chinese Hamster Ovary (CHO) cell lines, which showed cytotoxicity with IC50 value of 2.66 μg/mL.


Nickel oxide nanoparticles, Syzygium cumini, Antibacterial activity, Antifungal activity, Cytotoxic activity.

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