In vitro Cytotoxic Activity against MCF-7 Breast Cancer Cells Line: Pregabalin and Chiral Amino Acid-based Peptides

V.B. Pansuriya1, P.V. Dholaria2, S.L. Rathod1, U.B. Prajapati1, B.M. Vavaiya3, V.H. Marvaniya4, F.U. Vaidya5, C. Pathak5 and H.M. Parekh1,*,

1Department of Chemistry, School of Sciences, Gujarat University, Ahmedabad-380009, India

2BRCC Laboratory, Department of Chemistry, School of Science, R K University, Rajkot-360020, India

3Department of Chemistry, M.G. Science College, Ahmedabad-380009, India

4Shree Swaminarayan Sanskar Pharmacy College, Zundal, Gandinagar-382421, India

5Department of Cell Biology, School of Biological Sciences and Biotechnology, Indian Institute of Advanced Research, Gandhinagar-382426, India

*Corresponding author: E-mail: hiteshparekh@gujaratuniversity.ac.in

Abstract

Because of the medicinal application of peptides in the current drug discovery process, we report the synthesis and in vitro anticancer activity of various pregabalin containing peptides using chiral amino acids. The structures of newly synthesized compounds were assigned based on IR, 1H NMR, 13C NMR and mass spectral analysis. Synthesized peptides were screened for their anticancer activity on human breast cancer MCF-7 cells line by MMT assays method. Evaluation of anticancer activity shown that compound 13a (16.65% cell proliferation) was found to be most active against selected MCF-7 cell line.

Keywords

Peptide synthesis, Pregabalin, Cytotoxicity, Breast cancer, MCF-7 cell line.

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