Fractionation of Sugarcane Extracts and Determination of Phenolic Compounds and their Antioxidant Activity

K. Kerdchan1, and P. Srihanam2,*,

1Faculty of Pharmacy, Mahasarakham University, Maha Sarakham 44150, Thailand

2The Center of Excellence Innovation in Chemistry (PERCH-CIC) and Creative and Innovation Chemistry Research Unit, Department of Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand

*Corresponding author: Tel./Fax: +66 43754246; E-mail: prasong.s@msu.ac.th

Abstract

Crude ethanolic extracts of four cultivars of sugarcane (Saccharum officinarum L.) were firstly prepared and then fractionated using silica gel column chromatography before the determination of their oxidative substances. The fractionated extracts were then tested for antioxidant activity by various assays. Finally, high-performance liquid chromatography (HPLC) was applied for the quantitative determination of the individual phenolic compounds. Sub-fraction 2 has the highest phytochemical contents as well as antioxidant activity. All tested phytochemicals had positively correlated to antioxidant activity. HPLC analysis showed that the phytochemicals in the fractionated extracts varied by the sugarcane cultivars. The main flavonoid substances found in the fractionated extracts were epicatechin, catechin, quercetin, resveratrol, myricetin and rutin while gallic acid was the main phenolic substance. The obtained information is useful for further studies and applications.

Keywords

Partial purification, Fraction, Phytochemicals, Silica gel, Antioxidant activity.

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