Biosynthesis and Characterization of Bioplastic Poly-3-hydroxybutyrate from Hydrolyzate of Ulva using Bacillus subtilis rnM

T. Revadhi*, and R. Nanthini

Post Graduate and Research Department of Chemistry, Pachaiyappa’s College, Chennai-600030, India

*Corresponding author: E-mail:


Biopolymers are a new generation biopolymers, which have wide range of applications. Poly-3-hydroxybutyrate (PHB) is one of the best biopolymers for replacement of non-biodegradable petroleum-based plastic and can be produced from organic wastes source by some bacterial strains under imbalance growth conditions. In present study, hydrolyzate was prepared from biomass of seaweed Ulva by acid pre-treated and used as a feedstock for PHB biosynthesis by Bacillu subtilis rnM. The pre-treatment was carried out by two different pre-treatment conditions such as room temperature and high temperature and pressure (HTP). The hydrolysate prepared by 2 % HCl at HTP supported for the maximum PHB biosynthesis than the other pre-treated conditions. The yield of PHB obtained by B. subtilis rnM when tested with laboratory grade sugars was lower to that achieved with hydrolysate of Ulva sp. The biosynthesized PHB was characterized by TGA, DSC, FTIR, XRD and NMR techniques.


Poly-3-hydroxybutyrate, Bacillus subtilis rnM, Hydrolyzate, Ulva.

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