Protease Producers Predominate Cultivable Hydrolytic Bacteria Isolated from Liquid Biomedical Waste

Stalis Norma Ethica1,*, Sakti Imam Muchlissin2, Ragil Saptaningtyas1 and Agus Sabdono2

1Faculty of Nursing and Health Sciences, Universitas Muhammadiyah Semarang, Semarang, Indonesia

2Faculty of Fisheries and Marine Sciences, Universitas Diponegoro, Semarang, Indonesia

*Corresponding author: E-mail:


Hydrolytic bacteria are known for their crucial role in the bioconversion of organic matters mainly present in biomedical waste, making them advantageous in bioremediation. Analysis of abundance and colony diversity of culturable bacteria producing hydrolytic enzymes isolated from liquid biomedical waste of two different classes of hospitals in Semarang city, Central Java was carried out. Bacterial cultivation on nutrient agar slants resulted in total 26 viable colonies from inlet samples of Rumah Sakit Umum Roemani Muhammadiyah (R1) and Rumah Sakit Umum Daerah KRMT Wongsonegoro (R2) hospitals. Both hospitals represented two different classes of hospitals in Semarang (C and B, where B class is higher grade). Screening for production of four main hydrolytic enzymes (protease, lipase, amylase and cellulose) on all cultivable colonies obtained in this study. The results showed that all of them were capable of producing more than one type of hydrolytic enzymes. The composition was as follows: Protease producers (26 colonies), amylase producers (14 colonies), cellulose producers (13 colonies) and lipase producers (10 colonies).This study demonstrated that primary inlet of hospitals is a rich source of hydrolytic bacteria, where extracellular protease producers were found to be the main population of this group. In Semarang case, the inlet of lower class of hospital appeared to be more potential source of these hydrolytic bacteria than that of higher class one.


Protease producers, Clinical waste, Cultivable hydrolytic bacteria, Colony viability, Semarang hospital.

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