Nurhayati Bialangi¹, Adam Mustapa², Yusda Salimi¹, Ari Widiantoro³ and Boima Situmeang^4,*

¹Department of Chemistry, Faculty of Mathematic and Natural Sains, State University of Gorontalo, Gorontalo 96128, Indonesia

²Department of Pharmacy, Faculty of Sport and Healthy, State University of Gorontalo, Gorontalo 96128, Indonesia

³Department of Chemistry, Faculty of Mathematics and Natural Sains, Tanjungpura University, Kota Pontianak, Kalimantan Barat 78115, Indonesia

⁴Department of Chemistry, Sekolah Tinggi Analis Kimia Cilegon, Banten 42411, Indonesia

*Corresponding author: E-mail: boimatumeang@gmail.com

Suruhan (Peperomia pellucida) is easily found in Indonesia and has the potential of being a herb medicine. General phytochemical screening revealed the presence of flavonoid, steroid, triterpenoid, alkaloid and tannin compounds. Two steroid compounds were isolated from ethyl acetate fraction of the Peperomia pellucida. The compounds were isolated with chromatography method and antimalarial activity test with Desjardin method. Based on the spectral evidence IR, ¹H NMR, ¹³C NMR, 2D NMR and MS spectroscopies, structures were determined to be stigmasterol (1) and fucosterol (2). The results of antimalarial activity test showed IC₅₀ value of stigmasterol is 5.24 ppm and fucosterol is 0.85 ppm. This is the first report so far of isolation of steroid compounds from Peperomia pellucida plant from Gorontalo, Indonesia and their antimalarial activity test.

Suruhan, Steroid, Antimalarial, Peperomia pellucida.