HPLC-MS/MS Based Profiling of Antimutagenic Agents of Digera muriacata Leaf

Muhammad Yasir1, Bushra Sultana1,*, Haq Nawaz Bhatti1 and Muhammad Shahid2

1Department of Chemistry, University of Agriculture, Faisalabad-38040, Pakistan

2Department of Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan

*Corresponding author: E-mail: bushrasultana2005@yahoo.com

Abstract

The current study was aimed to characterize antimutagenic agents of Digera muriacata by high performance liquid chromatography coupled with mass spectrometry. Extraction procedure was optimized for better recovery of antimutagenic agents using acidified methanol (0.5, 1.0 and 2.0 N) and aqueous methanol (70 %, 50 % and 30 % v/v) to get hydrolyzed and non-hydrolyzed extracts, respectively. Both types of extracts were found to be non-mutagens with hydrolyzed extracts depicting higher antimutagenicity (70.63 ± 0.69 % for TA98 and 73.98 ± 0.70 % for TA100) than those of non-hydrolyzed extracts (68.25 ± 0.69 % for TA98 and 68.29 ± 1.22 % for TA100). Hence, optimized hydrolyzed extract was further characterized for its bioactives by LC-MS/MS analysis which showed the presence of ten compounds including kaempferol-O-dirhamnoside, 5-O-feruloylquinic acid, malvidine-3-O-glucoside, etc. These results potentially recommended vast applications of Digera muriacata in medicinal formulations for curing various diseases.

Keywords

Digera muriacata, LCMS/MS, Antimutagenic activity.

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