Analysis of the Chemical Constituents of BaZhen Decoction in Rat Urine by HPLC-ESI/MS

Wenlan Li1,2,*, Jing Bai1,2, Qichang Dai1,2, Yan Zhang1,2 and Yubin Ji1,2

1Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 50076, P.R. China

2Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin 150076, P.R. China

*Corresponding author: E-mail:;


High performance liquid chromatography/mass spectrometry (HPLC-ESI/MS) method was used to study BaZhen decoction and its metabolites in rat urine. 30 of major fragment ion peaks were marked in the total ion chromatogram of urine containing BaZhen decoction. According to the molecular weight and characteristic fragmentation information, 20 peaks were identified, containing 7 prototype constituents: Glycyol, glyuranolide, paeoniflorin, ginsenoside Rb2, liquiritin, PGG, ginsenoside Rd; and 13 metabolites: p-Hydroxycinnamic acid, 5-FDCA, pinicolic acid, 3a-hydroxyglycyrrhehetic acid, paeonimetabolin I, M1, Compound K, M2, tumulosic acid, M3, n-g-hydroxy-butyl phthalide, M4, Ginsenoside Rh1. The result shows that the final metabolites in rat urine of BaZhen decoction, which is quite valuable for the further studies on effective constituents and the effecting mechanism as well as for clinical application of Bazhen decoction.


BaZhen decoction, HPLC-ESI/MS, Chemical constituents, Metabolites, Urine.

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