Quantitative Determination of Triterpenoidal Saponins in Platycodi radix and Its Variation in Different Regions of Korean Peninsula: A Herbal Plant Used as Traditional Medicine

S.-J. Kim1,*, M. Valan Arasu1, N.A. Al-Dhabi2, D.-S. Yoo3, M.-H. Park4, Y.-S. Shin5 and S.-W. Lee5,*

1Department of Biological Environment and Chemistry, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Republic of Korea

2Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

3Korea Research Institute of Chemical Technology, Daejeon 305-600, Republic of Korea

4Department of Horticultural Crop Research, National Institute of Horticultural and Herbal Science (NIHHS), RDA, 475 Imok-dong, Jangan-gu, Suwon 440-706, Republic of Korea

5Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA, Eumseong 369-873, Republic of Korea

*Corresponding author: Fax: +82 42 8217142; Tel: +82 42 8216738; E-mail: kimsunju@cnu.ac.kr; swlee1004@korea.kr

Abstract

This survey was undertaken in different provinces in order to evaluate the contents of triterpenoidal saponins in Platycodi radix and its variation in different regions of Korea. HPLC and ESI-MS analysis were conducted for identification and quantification of ten know and four unknown compounds. Fourteen saponin compounds such as deapio-platycoside E, platycoside E, platycodin D3, platyconic acid A, platycodin D2, platycodin D, mixture of polygalacin D2 and 3'-O-acetyl-platycodin D, polygalacin D, 3'-O-acetyl-polygalacin D, 2'-O-acetyl-platycodin D and four unknown compounds widely ranged from 2,643 to 23,599 mg/kg dry weight (DW) in the 3-year-old roots and from 5,693 to 34,205 mg/kg DW in the 2-year-old roots, respectively. Since KFDA has not assigned any individual compounds for the quality control of Platycodi radix, we would suggest using Platycodi radix from the region of Jinan because the samples contain moderate total saponins contents.

Keywords

Platycodi radix, Platycodin D, Triterpenoidal saponins, HPLC-ELSD, LC/ESI-MS.

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