Microwave-Assisted Extraction and Nitric oxide and Superoxide Attenuation Potential of Polyphenolics from Bauhinia variegata

Shumaila Anwar, Nazish Jahan*, Khalil-Ur-Rahman, Shaukat Ali and Saba Aslam

Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, Pakistan

*Corresponding author: E-mail: nazishjahanuaf@yahoo.com

Abstract

An emerging microwave-assisted extraction has many advantages over conventionally used extraction techniques for the extraction of antioxidants. This research had been designed to investigate the efficiency of microwave assisted technique for the extraction of antioxidant polyphenols from different parts of Bauhinia variegata. Polyphenols were extracted by conventional (soxhlet, reflux) and emerging techniques (microwave-assisted extraction method) by employing solvents of different polarity. Total phenolic contents were measured by Folin-Ciocalteu method. Highest total phenolic contents were observed in the stem extract of B. variegata using Soxhlet technique but microwave-assisted extraction was found to be the fastest method for the extraction of phenolic contents. Nitric oxide and superoxide attenuation potential were evaluated by nitric oxide and super oxide radical attenuation assays. DPPH attenuation, reducing power, antioxidant activity in linoleic acid was also evaluated. Highest antioxidant potential was found in stem bark extract of B. variegata followed by flower and leave.

Keywords

Microwave-assisted extraction, Antioxidant potential, Nitric oxide attenuation potential, Super oxide attenuation potential.

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