Spectrophotometric Determination of Mesna in Commercial Injections

Wei-Xing Ma1,2,*, Na Li1, Ou Sha1 and Ying-Hong Liu1

1School of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, Jiangsu Province, P.R. China

2Jiangsu-Marine Resources Development Research Institute, Lianyungang 222005, Jiangsu Province, P.R. China

*Corresponding author: Tel: +86 518 85895405; E-mail: wxma650528@hhit.edu.cn; weixingma650528@163.com


A new visible spectrophotometric method for the determination of mesna was carried out based on the reaction of ferric solution with mesna to produce ferriferrous ion. The dependence of the absorbance on pH, temperature and reaction time was investigated. In Clark-lubs buffer medium, the red complex with the absorbance decreased obviously at 480 nm. The decrease value of the absorbance (DA), due to the presence of mesna was correlated with its concentration. Linear relationship with good correlation coefficient (0.9991) was found between DA and the concentration of mesna in a concentration range of 1-30 μg mL-1. The assay limit of determination was 0.31 μg mL-1. The proposed method was successfully applied to the determination of the investigated drug in injections with the recoveries of the range from 99.4 to 100.2 %.


Mesna, Spectrophotometry, Injection.

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  • Asian J. Chem. /
  •  2011 /
  •  23(8) /
  •  pp 3522-3524